Plasmid

Part:BBa_K4213013:Design

Designed by: Ioannis Retalis   Group: iGEM22_Thessaly   (2022-08-27)


pENTR-GFP-aHHR_theophylline


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1496
    Illegal XbaI site found at 1670
    Illegal SpeI site found at 2412
    Illegal PstI site found at 2390
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1496
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NheI site found at 721
    Illegal NheI site found at 2404
    Illegal SpeI site found at 2412
    Illegal PstI site found at 2390
    Illegal NotI site found at 671
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1496
    Illegal BamHI site found at 1650
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1496
    Illegal XbaI site found at 1670
    Illegal SpeI site found at 2412
    Illegal PstI site found at 2390
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1496
    Illegal XbaI site found at 1670
    Illegal SpeI site found at 2412
    Illegal PstI site found at 2390
    Illegal NgoMIV site found at 2141
    Illegal AgeI site found at 730
    Illegal AgeI site found at 2014
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 116


Design Notes

Not yet


Source

Dr.Dietmar Funck

References